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Applied and Environmental Microbiology, November 2005, p. 7607-7609, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7607-7609.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Creating New Genes by Plasmid Recombination in Escherichia coli and Bacillus subtilis

Ana Gomez,2 Tatjana Galic,2,{dagger} Jean-François Mariet,2 Ivan Matic,1 Miroslav Radman,1 and Marie-Agnès Petit1*

U571 INSERM Faculté de Médecine, Necker-Enfants Malades, 156 rue de Vaugirard, 75015 Paris, France,1 Eucodis GmbH, Brunner Strasse 59, A-1230 Wien, Austria2

Received 10 May 2005/ Accepted 16 June 2005

Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.

* Corresponding author. Mailing address: URLGA, INRA, 78352 Jouy en Josas, France. Phone: 33 1 34 65 28 67. Fax: 33 1 34 65 25 22. E-mail: marie-agnes.petit{at}jouy.inra.fr.

{dagger} Present address: PLIVA, Prilaz Baruna Filipovica 29, 10000 Zagreb, Croatia.

Applied and Environmental Microbiology, November 2005, p. 7607-7609, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7607-7609.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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