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Applied and Environmental Microbiology, December 2005, p. 8236-8240, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8236-8240.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cost-Effective Application of Pulsed-Field Gel Electrophoresis to Typing of Salmonella enterica Serovar Typhimurium

Geraldine Doran,1* Dearbhaile Morris,1 Colette O'Hare,1 Niall DeLappe,1 Bernard Bradshaw,3 Geraldine Corbett-Feeney,1,2 and Martin Cormican1,2

National Salmonella Reference Laboratory, University College Hospital, Galway, Ireland,1 Department of Bacteriology, National University of Ireland, Galway, Ireland,2 Bacteriology Division, Central Veterinary Research Laboratory, Dublin, Ireland3

Received 18 April 2005/ Accepted 27 August 2005

Salmonella enterica serovar Typhimurium is frequently isolated from humans and animals. Phage typing is historically the first-line reference typing technique in Europe. It is rapid and convenient for laboratories with appropriate training and experience, and costs of consumables are low. Phage typing and pulsed-field gel electrophoresis (PFGE) were performed on 503 isolates of serovar Typhimurium. Twenty-nine phage types and 53 PFGE patterns were observed. Most isolates of phage types DT104, DT104b, and U310 are not distinguishable from other members of their phage type by PFGE. By contrast, PFGE of isolates of phage types DT193 and U302 shows great heterogeneity. Analysis of experience with PFGE and phage typing can facilitate the selective application of PFGE to maximize the yield of epidemiologically relevant additional information while controlling costs.

* Corresponding author. Mailing address: National Salmonella Reference Laboratory, University College Hospital, Galway, Ireland. Phone: 353 91 544628. Fax: 353 91 512514. E-mail: Geraldine.Doran{at}nuigalway.ie.

Applied and Environmental Microbiology, December 2005, p. 8236-8240, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8236-8240.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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Copyright © 2005 by the American Society for Microbiology. All rights reserved.