This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buck, B. L.
Right arrow Articles by Klaenhammer, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buck, B. L.
Right arrow Articles by Klaenhammer, T. R.
Agricola
Right arrow Articles by Buck, B. L.
Right arrow Articles by Klaenhammer, T. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2005, p. 8344-8351, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8344-8351.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Analysis of Putative Adhesion Factors in Lactobacillus acidophilus NCFM

B. Logan Buck,1,2 Eric Altermann,1 Tina Svingerud,1 and Todd R. Klaenhammer1,2*

Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, North Carolina,1 Department of Microbiology, North Carolina State University, Raleigh, North Carolina2

Received 19 August 2005/ Accepted 22 August 2005

Lactobacilli are major inhabitants of the normal microflora of the gastrointestinal tract, and some select species have been used extensively as probiotic cultures. One potentially important property of these organisms is their ability to interact with epithelial cells in the intestinal tract, which may promote retention and host-bacterial communication. However, the mechanisms by which they attach to intestinal epithelial cells are unknown. The objective of this study was to investigate cell surface proteins in Lactobacillus acidophilus that may promote attachment to intestinal tissues. Using genome sequence data, predicted open reading frames were searched against known protein and protein motif databases to identify four proteins potentially involved in adhesion to epithelial cells. Homologous recombination was used to construct isogenic mutations in genes encoding a mucin-binding protein, a fibronectin-binding protein, a surface layer protein, and two streptococcal R28 homologs. The abilities of the mutants to adhere to intestinal epithelial cells were then evaluated in vitro. Each strain was screened on Caco-2 cells, which differentiate and express markers characteristic of normal small-intestine cells. A significant decrease in adhesion was observed in the fibronectin-binding protein mutant (76%) and the mucin-binding protein mutant (65%). A surface layer protein mutant also showed reduction in adhesion ability (84%), but the effect of this mutation is likely due to the loss of multiple surface proteins that may be embedded in the S-layer. This study demonstrated that multiple cell surface proteins in L. acidophilus NCFM can individually contribute to the organism's ability to attach to intestinal cells in vitro.

* Corresponding author. Mailing address: Department of Food Science, North Carolina State University, Box 7624, Raleigh, NC 27695. Phone: (919) 515-2972. Fax: (919) 515-7124. E-mail: klaenhammer{at}ncsu.edu.

Applied and Environmental Microbiology, December 2005, p. 8344-8351, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8344-8351.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ryan, K. A., O'Hara, A. M., van Pijkeren, J.-P., Douillard, F. P., O'Toole, P. W. (2009). Lactobacillus salivarius modulates cytokine induction and virulence factor gene expression in Helicobacter pylori. J Med Microbiol 58: 996-1005 [Abstract] [Full Text]  
  • Goh, Y. J., Azcarate-Peril, M. A., O'Flaherty, S., Durmaz, E., Valence, F., Jardin, J., Lortal, S., Klaenhammer, T. R. (2009). Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM. Appl. Environ. Microbiol. 75: 3093-3105 [Abstract] [Full Text]  
  • Azcarate-Peril, M. A., Tallon, R., Klaenhammer, T. R. (2009). Temporal gene expression and probiotic attributes of Lactobacillus acidophilus during growth in milk. J DAIRY SCI 92: 870-886 [Abstract] [Full Text]  
  • Prado Acosta, M., Palomino, M. M., Allievi, M. C., Rivas, C. S., Ruzal, S. M. (2008). Murein Hydrolase Activity in the Surface Layer of Lactobacillus acidophilus ATCC 4356. Appl. Environ. Microbiol. 74: 7824-7827 [Abstract] [Full Text]  
  • Konstantinov, S. R., Smidt, H., de Vos, W. M., Bruijns, S. C. M., Singh, S. K., Valence, F., Molle, D., Lortal, S., Altermann, E., Klaenhammer, T. R., van Kooyk, Y. (2008). S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions. Proc. Natl. Acad. Sci. USA 105: 19474-19479 [Abstract] [Full Text]  
  • Sela, D. A., Chapman, J., Adeuya, A., Kim, J. H., Chen, F., Whitehead, T. R., Lapidus, A., Rokhsar, D. S., Lebrilla, C. B., German, J. B., Price, N. P., Richardson, P. M., Mills, D. A. (2008). The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proc. Natl. Acad. Sci. USA 105: 18964-18969 [Abstract] [Full Text]  
  • Lebeer, S., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiol. Mol. Biol. Rev. 72: 728-764 [Abstract] [Full Text]  
  • Azcarate-Peril, M. A., Altermann, E., Goh, Y. J., Tallon, R., Sanozky-Dawes, R. B., Pfeiler, E. A., O'Flaherty, S., Buck, B. L., Dobson, A., Duong, T., Miller, M. J., Barrangou, R., Klaenhammer, T. R. (2008). Analysis of the Genome Sequence of Lactobacillus gasseri ATCC 33323 Reveals the Molecular Basis of an Autochthonous Intestinal Organism. Appl. Environ. Microbiol. 74: 4610-4625 [Abstract] [Full Text]  
  • Callanan, M., Kaleta, P., O'Callaghan, J., O'Sullivan, O., Jordan, K., McAuliffe, O., Sangrador-Vegas, A., Slattery, L., Fitzgerald, G. F., Beresford, T., Ross, R. P. (2008). Genome Sequence of Lactobacillus helveticus, an Organism Distinguished by Selective Gene Loss and Insertion Sequence Element Expansion. J. Bacteriol. 190: 727-735 [Abstract] [Full Text]  
  • Pfeiler, E. A., Azcarate-Peril, M. A., Klaenhammer, T. R. (2007). Characterization of a Novel Bile-Inducible Operon Encoding a Two-Component Regulatory System in Lactobacillus acidophilus. J. Bacteriol. 189: 4624-4634 [Abstract] [Full Text]  
  • Klaenhammer, T. R., Azcarate-Peril, M. A., Altermann, E., Barrangou, R. (2007). Influence of the Dairy Environment on Gene Expression and Substrate Utilization in Lactic Acid Bacteria. J. Nutr. 137: 748S-750S [Abstract] [Full Text]  
  • Boekhorst, J., Wels, M., Kleerebezem, M., Siezen, R. J. (2006). The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment.. Microbiology 152: 3175-3183 [Abstract] [Full Text]  
  • van Pijkeren, J.-P., Canchaya, C., Ryan, K. A., Li, Y., Claesson, M. J., Sheil, B., Steidler, L., O'Mahony, L., Fitzgerald, G. F., van Sinderen, D., O'Toole, P. W. (2006). Comparative and Functional Analysis of Sortase-Dependent Proteins in the Predicted Secretome of Lactobacillus salivarius UCC118.. Appl. Environ. Microbiol. 72: 4143-4153 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»