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Applied and Environmental Microbiology, December 2005, p. 8934-8936, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8934-8936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Detection of Mycobacterium avium subsp. paratuberculosis in Retail Cheeses from Greece and the Czech Republic

John Ikonomopoulos,1* Ivo Pavlik,2 Milan Bartos,2 Petra Svastova,2 Wuhib Yayo Ayele,2 Petr Roubal,3 John Lukas,4 Nigel Cook,5 and Maria Gazouli1

School of Animal Science, Department of Anatomy-Physiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece,1 Veterinary Research Institute, Hudcova 70, 62132 Brno, Czech Republic,2 Dairy Research Institute, Ke dvoru 12, 16000 Prague, Czech Republic,3 Department of Pharmacology, School of Medicine, Universidad del Pais Vasco, Leioa, Vizcaya, Spain,4 Central Science Laboratory, Sand Hutton YO41 1LZ, York, United Kingdom5

Received 26 April 2005/ Accepted 23 July 2005

We investigated the presence of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic. We found that 31.7% and 3.6% of our samples reacted positive by PCR and culture, respectively. Consumption of these cheeses is likely to result in human exposure to M. avium subsp. paratuberculosis, albeit at a low level for viable cells.


* Corresponding author. Mailing address: School of Animal Science, Department of Anatomy-Physiology, Agricultural University of Athens, Iera Odos 75, 11855 Votanikos, Athens, Greece. Phone: 302107510321. Fax: 302105294388. E-mail: ikonomop{at}aua.gr.


Applied and Environmental Microbiology, December 2005, p. 8934-8936, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8934-8936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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