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Applied and Environmental Microbiology, December 2005, p. 8944-8948, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8944-8948.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Legionella Confirmation Using Real-Time PCR and SYTO9 Is an Alternative to Current Methodology

Steven Giglio,* Paul T. Monis, and Christopher P. Saint

Australian Water Quality Centre, South Australian Water Corporation, Salisbury, South Australia 5108, Australia

Received 20 April 2005/ Accepted 8 August 2005

The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.

* Corresponding author. Mailing address: Australian Water Quality Centre, PMB 3, Salisbury, South Australia 5108, Australia. Phone: 61 8 82590312. Fax: 61 8 8259 0228. E-mail: steven.giglio{at}sawater.com.au.

Applied and Environmental Microbiology, December 2005, p. 8944-8948, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8944-8948.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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