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Applied and Environmental Microbiology, December 2005, p. 8949-8953, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8949-8953.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Analysis of fae and fhcD Genes in Mono Lake, California

Olivier Nercessian,1,{dagger} Marina G. Kalyuzhnaya,1 Samantha B. Joye,2 Mary E. Lidstrom,1,3 and Ludmila Chistoserdova1*

Department of Chemical Engineering,1 Department of Microbiology, University of Washington, Seattle, Washington 98195,3 Department of Marine Sciences, University of Georgia, Athens, Georgia 306022

Received 3 June 2005/ Accepted 11 August 2005

Genes for two enzymes of the tetrahydromethanopterin-linked C1 transfer pathway (fae and fhcD) were detected in hypersaline, hyperalkaline Mono Lake (California), via PCR amplification and analysis. Low diversity for fae and fhcD was noted, in contrast to the diversity previously detected in a freshwater lake, Lake Washington (Washington).

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Washington, Seattle, WA 98195. Phone: (206) 543-6683. Fax: (206) 616-5721. E-mail: milachis{at}u.washington.edu.

{dagger} Present address: CEA/Cadarache, DSV/DEVM/LEMiR, Bât 161, 13108 St. Paul lez Durance, France.

Applied and Environmental Microbiology, December 2005, p. 8949-8953, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8949-8953.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Kalyuzhnaya, M. G., Zabinsky, R., Bowerman, S., Baker, D. R., Lidstrom, M. E., Chistoserdova, L. (2006). Fluorescence In Situ Hybridization-Flow Cytometry-Cell Sorting-Based Method for Separation and Enrichment of Type I and Type II Methanotroph Populations.. Appl. Environ. Microbiol. 72: 4293-4301 [Abstract] [Full Text]  


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