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Applied and Environmental Microbiology, December 2005, p. 8954-8957, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8954-8957.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Differential Real-Time PCR Assay for Enumeration of Lactic Acid Bacteria in Wine

Ezekiel T. Neeley,{dagger} Trevor G. Phister,{ddagger} and David A. Mills*

Department of Viticulture & Enology, University of California, Davis, California 95616

Received 15 April 2005/ Accepted 15 August 2005

Oenococcus oeni is often employed to perform the malolactic fermentation in wine production, while nonoenococcal lactic acid bacteria often contribute to wine spoilage. Two real-time PCR assays were developed to enumerate the total, and nonoenococcal, lactic acid bacterial populations in wine. Used together, these assays can assess the spoilage risk of juice or wine from lactic acid bacteria.

* Corresponding author. Mailing address: Department of Viticulture and Enology, University of California, One Shields Ave., Davis, CA 95616. Phone: (530) 754-7821. Fax: (530) 752-0382. E-mail: damills{at}ucdavis.edu.

{dagger} Present address: Bonny Doon Vineyards, Santa Cruz, Calif.

{ddagger} Present address: Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pa.

Applied and Environmental Microbiology, December 2005, p. 8954-8957, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8954-8957.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Phister, T. G., Rawsthorne, H., Joseph, C.M. L., Mills, D. A. (2007). Real-Time PCR Assay for Detection and Enumeration of Hanseniaspora Species from Wine and Juice. Am. J. Enol. Vitic. 58: 229-233 [Abstract] [Full Text]  


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