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Applied and Environmental Microbiology, December 2005, p. 8958-8962, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8958-8962.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Novel Primers Reveal Wider Diversity among Marine Aerobic Anoxygenic Phototrophs{dagger}

Natalya Yutin,1 Marcelino T. Suzuki,2 and Oded Béjà1*

Department of Biology, Technion—Israel Institute of Technology, Haifa 32000, Israel,1 Chesapeake Biological Laboratory, University of Maryland Center for Environmental Sciences, P.O. Box 38, Solomons, Maryland 206882

Received 7 June 2005/ Accepted 23 August 2005

Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.

* Corresponding author. Mailing address: Department of Biology, Technion—Israel Institute of Technology, Haifa 32000, Israel. Phone: (972) 4829 3961. Fax: (972) 4822 5153. E-mail: beja{at}techunix.technion.ac.il.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2005, p. 8958-8962, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8958-8962.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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