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Applied and Environmental Microbiology, December 2005, p. 8982-8986, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8982-8986.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility Testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria

Ingo Klare,1* Carola Konstabel,1 Sibylle Müller-Bertling,1 Rolf Reissbrodt,1 Geert Huys,2 Marc Vancanneyt,3 Jean Swings,2,3 Herman Goossens,4 and Wolfgang Witte1

Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany,1 Laboratory of Microbiology,2 BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium,3 Medical Microbiology, University of Antwerp, Wilrijk, Belgium4

Received 17 December 2004/ Accepted 9 August 2005

Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Lactococcus, and Bifidobacterium. A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with L-cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.

* Corresponding author. Mailing address: Robert Koch Institute, Wernigerode Branch, Burgstraße 37, D-38855 Wernigerode, Germany. Phone: 49 3943 679 247. Fax: 49 3943 679 207. E-mail: i.klare{at}rki.de.

Applied and Environmental Microbiology, December 2005, p. 8982-8986, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8982-8986.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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