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Applied and Environmental Microbiology, February 2005, p. 1079-1083, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1079-1083.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Isolation and Characterization of Temperate Bacteriophages of Clostridium difficile

Shan Goh,1* Thomas V. Riley,1,2 and Barbara J. Chang1

Microbiology, School of Biomedical and Chemical Sciences, Queen Elizabeth II Medical Centre, The University of Western Australia,1 Division of Microbiology and Infectious Diseases, The Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia, Australia2

Received 17 January 2004/ Accepted 8 September 2004

ABSTRACT

The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages {phi}C2, {phi}C5, {phi}C6, and {phi}C8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of {phi}C2 showed nucleotide homology to the sequenced C. difficile strain CD630.

* Corresponding author. Present address: Department of Microbiology, National University of Singapore, Blk MD4A, 5 Science Dr. 2, #05-03, Singapore 117597, Republic of Singapore. Phone: 65 6874 3278. Fax: 65 67766872. E-mail: micgs{at}nus.edu.sg.

Applied and Environmental Microbiology, February 2005, p. 1079-1083, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1079-1083.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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