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Applied and Environmental Microbiology, February 2005, p. 1084-1088, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1084-1088.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

Tatsuo Miyoshi,1 Teruki Iwatsuki,2 and Takeshi Naganuma1,2*

Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima,1 Tono Geoscience Center, Japan Nuclear Cycle Development Institute, Izumicho, Toki, Japan2

Received 8 June 2004/ Accepted 16 September 2004

ABSTRACT

A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-µm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-µm-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-µm-pore-size filters were largely affiliated with Betaproteobacteria.

* Corresponding author. Mailing address: Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan. Phone: 81-82-424-7986. Fax: 81-82-422-7916. E-mail: takn{at}hiroshima-u.ac.jp.

Applied and Environmental Microbiology, February 2005, p. 1084-1088, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1084-1088.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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