Applied and Environmental Microbiology, February 2005, p. 1109-1113, Vol. 71, No. 2
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.2.1109-1113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
High-Level Acetaldehyde Production in Lactococcus lactis by Metabolic Engineering
Roger S. Bongers,1*
Marcel H. N. Hoefnagel,1 and
Michiel Kleerebezem1
Wageningen Centre for Food Sciences and FNI Department, NIZO food research, Ede, The Netherlands1
Received 14 April 2004/
Accepted 21 September 2004
ABSTRACT
Efficient conversion of glucose to acetaldehyde is achieved by nisin-controlled overexpression of Zymomonas mobilis pyruvate decarboxylase (pdc) and Lactococcus lactis NADH oxidase (nox) in L. lactis. In resting cells, almost 50% of the glucose consumed could be redirected towards acetaldehyde by combined overexpression of pdc and nox under anaerobic conditions.
* Corresponding author. Mailing address: NIZO food research, Department of Flavour and Natural Ingredients, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-(0)318-659511. Fax: 31-(0)318-650400. E-mail: Roger.Bongers{at}nizo.nl.
Applied and Environmental Microbiology, February 2005, p. 1109-1113, Vol. 71, No. 2
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.2.1109-1113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.