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 Previous Article

Applied and Environmental Microbiology, February 2005, p. 1117-1121, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1117-1121.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid and Simple Quantification of Bacterial Cells by Using a Microfluidic Device

Chieko Sakamoto,1,{dagger} Nobuyasu Yamaguchi,1,{dagger} and Masao Nasu*

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan1

Received 15 May 2004/ Accepted 14 September 2004

ABSTRACT

This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

* Corresponding author. Mailing address: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8170. Fax: 81-6-6879-8174. E-mail: nasu{at}phs.osaka-u.ac.jp.

FOOTNOTES

{dagger} C.S. and N.Y. contributed equally to this work.

Applied and Environmental Microbiology, February 2005, p. 1117-1121, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1117-1121.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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