This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hutchison, M. L.
Right arrow Articles by Avery, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hutchison, M. L.
Right arrow Articles by Avery, S. M.
Agricola
Right arrow Articles by Hutchison, M. L.
Right arrow Articles by Avery, S. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2005, p. 691-696, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.691-696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Fate of Pathogens Present in Livestock Wastes Spread onto Fescue Plots

Mike L. Hutchison,* Lisa D. Walters, Tony Moore, D. John I. Thomas, and Sheryl M. Avery

Microbiological Research Division, Direct Laboratories Ltd., Wolverhampton, West Midlands, United Kingdom

Received 16 June 2004/ Accepted 13 September 2004

Fecal wastes from a variety of farmed livestock were inoculated with livestock isolates of Escherichia coli O157, Listeria monocytogenes, Salmonella, Campylobacter jejuni, and Cryptosporidium parvum oocysts at levels representative of the levels found in naturally contaminated wastes. The wastes were subsequently spread onto a grass pasture, and the decline of each of the zoonotic agents was monitored over time. There were no significant differences among the decimal reduction times for the bacterial pathogens. The mean bacterial decimal reduction time was 1.94 days. A range of times between 8 and 31 days for a 1-log reduction in C. parvum levels was obtained, demonstrating that the protozoans were significantly more hardy than the bacteria. Oocyst recovery was more efficient from wastes with lower dry matter contents. The levels of most of the zoonotic agents had declined to below detectable levels by 64 days. However, for some waste types, 128 days was required for the complete decline of L. monocytogenes levels. We were unable to find significant differences between the rates of pathogen decline in liquid (slurry) and solid (farmyard manure) wastes, although concerns have been raised that increased slurry generation as a consequence of more intensive farming practices could lead to increased survival of zoonotic agents in the environment.

* Corresponding author. Mailing address: Direct Laboratories Ltd., Microbiological Research Division, Wergs Road, Wolverhampton, West Midlands WV6 8TQ, United Kingdom. Phone: 44 1902 693285. Fax: 44 1902 693310. E-mail: Mike.Hutchison{at}directlabs.co.uk.

Applied and Environmental Microbiology, February 2005, p. 691-696, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.691-696.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • St-Pierre, C., Levallois, P., Gingras, S., Payment, P., Gignac, M. (2009). Risk of diarrhea with adult residents of municipalities with significant livestock production activities. J Public Health (Oxf) 31: 278-285 [Abstract] [Full Text]  
  • Berard, N. C., Holley, R. A., McAllister, T. A., Ominski, K. H., Wittenberg, K. M., Bouchard, K. S., Bouchard, J. J., Krause, D. O. (2009). Potential To Reduce Escherichia coli Shedding in Cattle Feces by Using Sainfoin (Onobrychis viciifolia) Forage, Tested In Vitro and In Vivo. Appl. Environ. Microbiol. 75: 1074-1079 [Abstract] [Full Text]  
  • Holley, R., Walkty, J., Blank, G., Tenuta, M., Ominski, K., Krause, D., Ng, L.-K. (2008). Examination of Salmonella and Escherichia coli Translocation from Hog Manure to Forage, Soil, and Cattle Grazed on the Hog Manure-treated Pasture. J. Environ. Qual. 37: 2083-2092 [Abstract] [Full Text]  
  • Lopez-Benavides, M. G., Williamson, J. H., Pullinger, G. D., Lacy-Hulbert, S. J., Cursons, R. T., Leigh, J. A. (2007). Field Observations on the Variation of Streptococcus uberis Populations in a Pasture-Based Dairy Farm. J DAIRY SCI 90: 5558-5566 [Abstract] [Full Text]  
  • Ufnar, J. A., Ufnar, D. F., Wang, S. Y., Ellender, R. D. (2007). Development of a Swine-Specific Fecal Pollution Marker Based on Host Differences in Methanogen mcrA Genes. Appl. Environ. Microbiol. 73: 5209-5217 [Abstract] [Full Text]  
  • Ravva, S. V., Korn, A. (2007). Extractable Organic Components and Nutrients in Wastewater from Dairy Lagoons Influence the Growth and Survival of Escherichia coli O157:H7. Appl. Environ. Microbiol. 73: 2191-2198 [Abstract] [Full Text]  
  • Furukawa, Y., Hasegawa, H. (2006). Response of spinach and komatsuna to biogas effluent made from source-separated kitchen garbage.. J. Environ. Qual. 35: 1939-1947 [Abstract] [Full Text]  
  • Xiao, L., Moore, J. E., Ukoh, U., Gatei, W., Lowery, C. J., Murphy, T. M., Dooley, J. S. G., Millar, B. C., Rooney, P. J., Rao, J. R. (2006). Prevalence and Identity of Cryptosporidium spp. in Pig Slurry.. Appl. Environ. Microbiol. 72: 4461-4463 [Abstract] [Full Text]  
  • Holley, R. A., Arrus, K. M., Ominski, K. H., Tenuta, M., Blank, G. (2006). Salmonella Survival in Manure-Treated Soils during Simulated Seasonal Temperature Exposure. J. Environ. Qual. 35: 1170-1180 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»