This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lunde, M. Articles by Nes, I. F. Search for Related Content PubMed PubMed Citation Articles by Lunde, M. Articles by Nes, I. F. Agricola Articles by Lunde, M. Articles by Nes, I. F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2005, p. 721-727, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.721-727.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effects of Diverse Environmental Conditions on LC3 Prophage Stability in Lactococcus lactis

Merete Lunde,^1* Are Halvor Aastveit,¹ Janet Martha Blatny,^1, and Ingolf F. Nes¹

Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Aas, Norway¹

Received 11 June 2004/ Accepted 20 September 2004

The effects of various growth conditions on spontaneous LC3 prophage induction in Lactococcus lactis subsp. cremoris IMN-C1814 was analyzed with a half fraction of a 4⁴ factorial experimental design. The four factors included in the study were nutrient availability, acidity, osmolarity, and temperature, each applied at four levels. These environmental factors are related to the fermentation processes in the dairy industry, in which bacteriophage attacks on sensitive starter strains are a constant threat to successful fermentation processes. The frequency of spontaneous LC3 induction was determined by quantitative analyses of restored DNA attachment sites (attB) on the bacterial chromosomes in a population of lysogenic cells. Statistical analysis revealed that all four environmental factors tested affected LC3 prophage stability and that the environmental factors were involved in interactions (interactions exist when the effect of one factor depends on the level of another factor). The spontaneous LC3 induction frequency varied from 0.08 to 1.76%. In general, the induction frequency remained at the same rate or decreased when level 1 to 3 of the four environmental factors was applied. At level 4, which generally gave the least favorable growth conditions, the induction frequency was either unchanged, decreased, or increased, depending on the type of stress. It appeared that the spontaneous induction frequency was independent of the growth behavior of the host. It was the environmental growth conditions that were the decisive factor in induction frequency.

Present address: Department of Protection, Norwegian Defence Research Establishment, Kjeller, Norway.