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Applied and Environmental Microbiology, February 2005, p. 741-745, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.741-745.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Active Transport and Accumulation of Iodide by Newly Isolated Marine Bacteria

Seigo Amachi,1 Yukako Mishima,1 Hirofumi Shinoyama,1 Yasuyuki Muramatsu,2 and Takaaki Fujii1*

Department of Bioresources Chemistry, Chiba University, Matsudo, Matsudo-shi, Chiba,1 Department of Chemistry, Gakushuin University, Mejiro, Toshima-ku, Tokyo, Japan2

Received 2 July 2004/ Accepted 3 September 2004

Iodide (I–)-accumulating bacteria were isolated from marine sediment by an autoradiographic method with radioactive 125I–. When they were grown in a liquid medium containing 0.1 µM iodide, 79 to 89% of the iodide was removed from the medium, and a corresponding amount of iodide was detected in the cells. Phylogenetic analysis based on 16S rRNA gene sequences indicated that iodide-accumulating bacteria were closely related to Flexibacter aggregans NBRC15975 and Arenibacter troitsensis, members of the family Flavobacteriaceae. When one of the strains, strain C-21, was cultured with 0.1 µM iodide, the maximum iodide content and the maximum concentration factor for iodide were 220 ± 3.6 (mean ± standard deviation) pmol of iodide per mg of dry cells and 5.5 x 103, respectively. In the presence of much higher concentrations of iodide (1 µM to 1 mM), increased iodide content but decreased concentration factor for iodide were observed. An iodide transport assay was carried out to monitor the uptake and accumulation of iodide in washed cell suspensions of iodide-accumulating bacteria. The uptake of iodide was observed only in the presence of glucose and showed substrate saturation kinetics, with an apparent affinity constant for transport and a maximum velocity of 0.073 µM and 0.55 pmol min–1 mg of dry cells–1, respectively. The other dominant species of iodine in terrestrial and marine environments, iodate (IO3–), was not transported.

* Corresponding author. Mailing address: Department of Bioresources Chemistry, Chiba University, 648 Matsudo, Matsudo-shi, Chiba 271-8510, Japan. Phone: 81-47-308-8866. Fax: 81-47-308-8866. E-mail: tfujii{at}faculty.chiba-u.jp.

Applied and Environmental Microbiology, February 2005, p. 741-745, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.741-745.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Crockford, S. J. (2009). Evolutionary roots of iodine and thyroid hormones in cell-cell signaling. Integr. Comp. Biol. 49: 155-166 [Abstract] [Full Text]  
  • Amachi, S., Kimura, K., Muramatsu, Y., Shinoyama, H., Fujii, T. (2007). Hydrogen Peroxide-Dependent Uptake of Iodine by Marine Flavobacteriaceae Bacterium Strain C-21. Appl. Environ. Microbiol. 73: 7536-7541 [Abstract] [Full Text]  


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