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Applied and Environmental Microbiology, February 2005, p. 811-816, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.811-816.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Low-Temperature Growth of Shewanella oneidensis MR-1

Randa Abboud,1 Radu Popa,1* Virginia Souza-Egipsy,1,2 Carol S. Giometti,3 Sandra Tollaksen,4 Jennifer J. Mosher,4 Robert H. Findlay,4 and Kenneth H. Nealson1

Department of Biological Sciences, University of Southern California, Los Angeles, California,1 Biosciences Division, Argonne National Laboratory, Argonne, Illinois,3 Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama,4 Centro de Astrobiologia CSIC-INTA, Centra Torrejón a Ajalvir, Torrejón de Ardoz, Madrid, Spain2

Received 28 April 2004/ Accepted 3 September 2004

Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of {approx}35°C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature ({approx}22°C) MR-1 grows with a doubling time of about 40 min, but when moved from 22°C to 3°C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of {approx}67 h. In comparison to cells grown at 22°C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22°C.

* Corresponding author. Mailing address: Department of Biological Sciences, 3651 Trousdale Pkwy., AHF 107, University of Southern California, Los Angeles, CA 90089-0371. Phone: (213) 821-2269. Fax: (213) 821-2269. E-mail: rpopa{at}usc.edu.

Applied and Environmental Microbiology, February 2005, p. 811-816, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.811-816.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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