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Applied and Environmental Microbiology, February 2005, p. 811-816, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.811-816.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Low-Temperature Growth of Shewanella oneidensis MR-1

Department of Biological Sciences, University of Southern California, Los Angeles, California,¹ Biosciences Division, Argonne National Laboratory, Argonne, Illinois,³ Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama,⁴ Centro de Astrobiologia CSIC-INTA, Centra Torrejón a Ajalvir, Torrejón de Ardoz, Madrid, Spain²

Received 28 April 2004/ Accepted 3 September 2004

Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of 35°C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature (22°C) MR-1 grows with a doubling time of about 40 min, but when moved from 22°C to 3°C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of 67 h. In comparison to cells grown at 22°C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22°C.

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