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Applied and Environmental Microbiology, February 2005, p. 987-992, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.987-992.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Protozoan Acanthamoeba polyphaga as a Potential Reservoir for Campylobacter jejuni

Diana Axelsson-Olsson,1 Jonas Waldenström,2 Tina Broman,3 Björn Olsen,4,5 and Martin Holmberg6*

Department of Clinical Microbiology, Kalmar County Hospital,1 Department of Biology and Environmental Science, University of Kalmar, Kalmar,4 Department of Animal Ecology, Lund University, Lund,2 FOI, The Swedish Defence Research Agency,3 Department of Infectious Diseases, Umeå University, Umeå,5 Department of Medical Sciences, Section for Infectious Diseases, Uppsala University Hospital, Uppsala, Sweden6

Received 12 February 2004/ Accepted 13 September 2004

We showed by a laboratory experiment that four different Campylobacter jejuni strains are able to infect the protozoan Acanthamoeba polyphaga. C. jejuni cells survived for longer periods when cocultured with amoebae than when grown in culture alone. The infecting C. jejuni cells aggregated in amoebic vacuoles, in which they were seen to be actively moving. Furthermore, a resuscitation of bacterial cultures that were previously negative in culturability tests was observed after reinoculation into fresh amoeba cultures. After spontaneous rupture of the amoebae, C. jejuni could be detected by microscopy and culturability tests. Our results indicate that amoebae may serve as a nonvertebrate reservoir for C. jejuni in the environment.

* Corresponding author. Mailing address: Department of Medical Science, Section for Infectious Diseases, Uppsala University Hospital, S-751 85 Uppsala, Sweden. Phone: 46-18-6115662. Fax: 46-18-6115650. E-mail: martin.holmberg{at}medsci.uu.se.

Applied and Environmental Microbiology, February 2005, p. 987-992, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.987-992.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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