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Applied and Environmental Microbiology, March 2005, p. 1130-1134, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1130-1134.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Immobilization of Escherichia coli Cells by Use of the Antimicrobial Peptide Cecropin P1{dagger}

Kalvin Gregory1 and Charlene M. Mello2*

Department of Chemistry, University of Kentucky, Lexington, Kentucky, and Macromolecular Sciences Team, U.S. Army Center, Natick, Massachusetts Research, Development and Engineering Command, Natick Soldier,1 2

Received 4 May 2004/ Accepted 7 October 2004

An immobilization scheme for bacterial cells is described, in which the antimicrobial peptide cecropin P1 was used to trap Escherichia coli K-12 and O157:H7 cells on microtiter plate well surfaces. Cecropin P1 was covalently attached to the well surfaces, and E. coli cells were allowed to bind to the peptide-coated surface. The immobilized cells were detected colorimetrically with an anti-E. coli antibody-horseradish peroxidase conjugate. Binding curves were obtained in which the signal intensities were dependent upon the cell concentration and upon the amount of peptide attached to the well surface. After normalization for the amount of peptide coupled to the surface and the relative binding affinity of the antibody for each strain, the binding data were compared, which indicated that there was a strong preference for E. coli O157:H7 over E. coli K-12. The cells could be immobilized reproducibly at pH values ranging from 5 to 10 and at ionic strengths up to 0.50 M.

* Corresponding author. Mailing address: Macromolecular Sciences Team, U.S. Army Research, Development and Engineering Command, Natick Soldier Center, Kansas St., Natick, MA 01760-5020. Phone: (508) 233-5825. Fax: (508) 233-4469. E-mail: Charlene.mello{at}us.army.mil.

{dagger} This document reports research undertaken at the University of Kentucky and the U.S. Army Research, Development and Engineering Command, Natick Soldier Center, Natick, Mass., and is no. NATICK/TP-03/058 in a series of papers approved for publication by the U.S. Army Research, Development and Engineering Command, Natick Soldier Center.

Applied and Environmental Microbiology, March 2005, p. 1130-1134, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1130-1134.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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