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Applied and Environmental Microbiology, March 2005, p. 1223-1230, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1223-1230.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Analysis of Three Plasmids from Lactobacillus plantarum

Richard van Kranenburg,^1,2, Natasa Golic,^1,2 Roger Bongers,^1,2 Rob J. Leer,^1,3 Willem M. de Vos,¹ Roland J. Siezen,^1,2,4 and Michiel Kleerebezem^1,2*

Wageningen Centre for Food Sciences, Wageningen,¹ NIZO food research, Ede,² TNO Nutrition, Zeist,³ University of Nijmegen, Nijmegen, The Netherlands⁴

Received 26 March 2004/ Accepted 29 September 2004

Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.

Present address: PURAC Biochem, Gorinchem, The Netherlands.

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