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Applied and Environmental Microbiology, March 2005, p. 1638-1641, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1638-1641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Environmental DNA Fragment Conferring Early and Increased Sporulation and Antibiotic Production in Streptomyces Species{dagger}

Asuncion Martinez,* Steven J. Kolvek, Joern Hopke, Choi Lai Tiong Yip,{ddagger} and Marcia S. Osburne{ddagger}

Sanofi-Aventis Cambridge Genomics Center, Cambridge, Massachusetts

Received 15 July 2004/ Accepted 15 October 2004

ABSTRACT

Here we describe the rep gene, isolated from an environmental DNA library, which when transformed into Streptomyces species resulted in increased production of secondary metabolites and accelerated sporulation. We show that Streptomyces lividans strains bearing rep are particularly useful as expression hosts for heterologous antibiotic production.

* Corresponding author. Present address: MIT 48-336b, Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139. Phone: (617) 253-2459. Fax: (617) 258-7009. E-mail: chon{at}mit.edu.

FOOTNOTES

{dagger} This paper is dedicated to the memory of Steven J. Kolvek, our colleague and friend.

{ddagger} Present address: ActivBiotics, Inc., Lexington, MA 02420.

Applied and Environmental Microbiology, March 2005, p. 1638-1641, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1638-1641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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