This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nijenhuis, I. Articles by Zinder, S. H. Search for Related Content PubMed PubMed Citation Articles by Nijenhuis, I. Articles by Zinder, S. H. Agricola Articles by Nijenhuis, I. Articles by Zinder, S. H.

SHORT REPORT

Characterization of Hydrogenase and Reductive Dehalogenase Activities of Dehalococcoides ethenogenes Strain 195

Ivonne Nijenhuis and Stephen H. Zinder^*

Department of Microbiology, Cornell University, Ithaca, New York

Received 4 June 2004/ Accepted 14 October 2004

ABSTRACT

Dehalococcoides ethenogenes strain 195 reductively dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to vinyl chloride and ethene using H₂ as an electron donor. PCE- and TCE-reductive dehalogenase (RD) activities were mainly membrane associated, whereas only about 20% of the hydrogenase activity was membrane associated. Experiments with methyl viologen (MV) were consistent with a periplasmic location for the RDs or a component feeding electrons to them. The protonophore uncoupler tetrachlorosalicylanilide did not inhibit reductive dechlorination in cells incubated with H₂ and PCE and partially restored activity in cells incubated with the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. Benzyl viologen or diquat (E^o' –360 mV) supported reductive dechlorination of PCE or TCE at rates comparable to MV (–450 mV) in cell extracts.

FOOTNOTES

Present address: Department of Bioremediation, UFZ Centre for Environmental Research Leipzig-Halle, D-04318 Leipzig, Germany.

This article has been cited by other articles:

• Fung, J. M., Morris, R. M., Adrian, L., Zinder, S. H. (2007). Expression of Reductive Dehalogenase Genes in Dehalococcoides ethenogenes Strain 195 Growing on Tetrachloroethene, Trichloroethene, or 2,3-Dichlorophenol. Appl. Environ. Microbiol. 73: 4439-4445 [Abstract] [Full Text]  
• Rahm, B. G., Morris, R. M., Richardson, R. E. (2006). Temporal Expression of Respiratory Genes in an Enrichment Culture Containing Dehalococcoides ethenogenes. Appl. Environ. Microbiol. 72: 5486-5491 [Abstract] [Full Text]