Applied and Environmental Microbiology, March 2005, p. 1664-1667, Vol. 71, No. 3
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.3.1664-1667.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Characterization of Hydrogenase and Reductive Dehalogenase Activities of Dehalococcoides ethenogenes Strain 195
Ivonne Nijenhuis
and
Stephen H. Zinder*
Department of Microbiology, Cornell University, Ithaca, New York
Received 4 June 2004/
Accepted 14 October 2004
ABSTRACT
Dehalococcoides ethenogenes strain 195 reductively dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to vinyl chloride and ethene using H2 as an electron donor. PCE- and TCE-reductive dehalogenase (RD) activities were mainly membrane associated, whereas only about 20% of the hydrogenase activity was membrane associated. Experiments with methyl viologen (MV) were consistent with a periplasmic location for the RDs or a component feeding electrons to them. The protonophore uncoupler tetrachlorosalicylanilide did not inhibit reductive dechlorination in cells incubated with H2 and PCE and partially restored activity in cells incubated with the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. Benzyl viologen or diquat (Eo'
360 mV) supported reductive dechlorination of PCE or TCE at rates comparable to MV (450 mV) in cell extracts.
* Corresponding author. Mailing address: Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-2415. Fax: (607) 255-3904. E-mail: shz1{at}cornell.edu.
FOOTNOTES
Present address: Department of Bioremediation, UFZ Centre for Environmental Research Leipzig-Halle, D-04318 Leipzig, Germany.
Applied and Environmental Microbiology, March 2005, p. 1664-1667, Vol. 71, No. 3
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.3.1664-1667.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.