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Applied and Environmental Microbiology, March 2005, p. 1671-1673, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1671-1673.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Application of a Newly Developed ARB Software-Integrated Tool for In Silico Terminal Restriction Fragment Length Polymorphism Analysis Reveals the Dominance of a Novel pmoA Cluster in a Forest Soil

Peter Ricke, Steffen Kolb, and Gesche Braker*

Max Planck Institute for Terrestrial Microbiology, Marburg, Germany

Received 28 July 2004/ Accepted 18 October 2004

ABSTRACT

TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool to perform directed terminal restriction fragment length polymorphism analysis of pmoA products obtained from a forest soil revealed that novel cluster I methanotrophic bacteria were dominant.

* Corresponding author. Mailing address: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany. Phone: 49 6421/178733. Fax: 49 6421/178999. E-mail: braker{at}staff.uni-marburg.de.

Applied and Environmental Microbiology, March 2005, p. 1671-1673, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1671-1673.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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