This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Compant, S.
Right arrow Articles by Ait Barka, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Compant, S.
Right arrow Articles by Ait Barka, E.
Agricola
Right arrow Articles by Compant, S.
Right arrow Articles by Ait Barka, E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2005, p. 1685-1693, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.1685-1693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Endophytic Colonization of Vitis vinifera L. by Plant Growth-Promoting Bacterium Burkholderia sp. Strain PsJN

Stéphane Compant,1 Birgit Reiter,2 Angela Sessitsch,2 Jerzy Nowak,3 Christophe Clément,1 and Essaïd Ait Barka1*

Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, Reims, France,1 Department of Bioresources, ARC Seibersdorf Research GmbH, Seibersdorf, Austria,2 Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia3

Received 10 June 2004/ Accepted 25 October 2004

Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA (PsJN::gusA11) genes were used to enumerate and visualize tissue colonization. The rhizospheres of 4- to 5-week-old plantlets with five developed leaves were inoculated with bacterial suspensions. Epiphytic and endophytic colonization patterns were then monitored by dilution plating assays and microscopic observation of organ sections. Bacteria were chronologically detected first on root surfaces, then in root internal tissues, and finally in the fifth internode and the tissues of the fifth leaf. Analysis of the PsJN colonization patterns showed that this strain colonizes grapevine root surfaces, as well as cell walls and the whole surface of some rhizodermal cells. Cells were also abundant at lateral root emergence sites and root tips. Furthermore, cell wall-degrading endoglucanase and endopolygalacturonase secreted by PsJN explained how the bacterium gains entry into root internal tissues. Host defense reactions were observed in the exodermis and in several cortical cell layers. Bacteria were not observed on stem and leaf surfaces but were found in xylem vessels of the fifth internode and the fifth leaf of plantlets. Moreover, bacteria were more abundant in the fifth leaf than in the fifth internode and were found in substomatal chambers. Thus, it seems that Burkholderia sp. strain PsJN induces a local host defense reaction and systemically spreads to aerial parts through the transpiration stream.

* Corresponding author. Mailing address: Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, 51687 Reims Cédex 2, France. Phone and fax: 33-3-26-91-34-41. E-mail: ea.barka{at}univ-reims.fr.

Applied and Environmental Microbiology, April 2005, p. 1685-1693, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.1685-1693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Onofre-Lemus, J., Hernandez-Lucas, I., Girard, L., Caballero-Mellado, J. (2009). ACC (1-Aminocyclopropane-1-Carboxylate) Deaminase Activity, a Widespread Trait in Burkholderia Species, and Its Growth-Promoting Effect on Tomato Plants. Appl. Environ. Microbiol. 75: 6581-6590 [Abstract] [Full Text]  
  • Verhagen, B. W. M., Trotel-Aziz, P., Couderchet, M., Hofte, M., Aziz, A. (2009). Pseudomonas spp.-induced systemic resistance to Botrytis cinerea is associated with induction and priming of defence responses in grapevine. J Exp Bot 0: erp295v1-erp295 [Abstract] [Full Text]  
  • Gambetta, G. A., Rost, T. L., Matthews, M. A. (2009). Passive Pathogen Movement via Open Xylem Conduits in Grapevine Graft Unions. Am. J. Enol. Vitic. 60: 241-245 [Abstract] [Full Text]  
  • Ait Barka, E., Nowak, J., Clement, C. (2006). Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium, Burkholderia phytofirmans Strain PsJN. Appl. Environ. Microbiol. 72: 7246-7252 [Abstract] [Full Text]  
  • CHATELET, D. S., MATTHEWS, M. A., ROST, T. L. (2006). Xylem Structure and Connectivity in Grapevine (Vitis vinifera) Shoots Provides a Passive Mechanism for the Spread of Bacteria in Grape Plants. ANN BOT (LOND) 98: 483-494 [Abstract] [Full Text]  
  • Thorne, E. T., Young, B. M., Young, G. M., Stevenson, J. F., Labavitch, J. M., Matthews, M. A., Rost, T. L. (2006). The structure of xylem vessels in grapevine (Vitaceae) and a possible passive mechanism for the systemic spread of bacterial disease. Am. J. Bot. 93: 497-504 [Abstract] [Full Text]  
  • Compant, S., Duffy, B., Nowak, J., Clement, C., Barka, E. A. (2005). Use of Plant Growth-Promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanisms of Action, and Future Prospects. Appl. Environ. Microbiol. 71: 4951-4959 [Full Text]  
  • Sessitsch, A., Coenye, T., Sturz, A. V., Vandamme, P., Barka, E. A., Salles, J. F., Van Elsas, J. D., Faure, D., Reiter, B., Glick, B. R., Wang-Pruski, G., Nowak, J. (2005). Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties. Int. J. Syst. Evol. Microbiol. 55: 1187-1192 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»