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Applied and Environmental Microbiology, April 2005, p. 2079-2085, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2079-2085.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Spatial Distribution and Transcriptional Activity of an Uncultured Clade of Planktonic Diazotrophic {gamma}-Proteobacteria in the Arabian Sea

Clare Bird,1 Joaquín Martinez Martinez,1,{dagger} Anthony G. O'Donnell,2 and Michael Wyman1*

School of Biological and Environmental Sciences, University of Stirling, Stirling,1 School of Biology, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom2

Received 29 July 2004/ Accepted 13 November 2004

The spatial distribution of an uncultured clade of marine diazotrophic {gamma}-proteobacteria in the Arabian Sea was investigated by the development of a specific primer pair to amplify an internal fragment of nifH by PCR. These organisms were most readily detected in highly oligotrophic surface waters but could also be found in deeper waters below the nutricline. nifH transcripts originating from this clade were detected in oligotrophic surface waters and, in addition, in the deeper and the more productive near-coastal waters. The nifH sequences most closely related to the unidentified marine bacterial group are from environmental clones amplified from the Atlantic and Pacific Oceans. These findings suggest that these {gamma}-proteobacteria are widespread and likely to be an important component of the heterotrophic diazotrophic microbial community of the tropical and subtropical oceans.

* Corresponding author. Mailing address: School of Biological and Environmental Sciences, University of Stirling, Stirling FK9 4LA United Kingdom. Phone: 44 1786 467844. Fax: 44 1786 464994. E-mail: michael.wyman{at}stirling.ac.uk.

{dagger} Present address: Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth PL1 3DH, United Kingdom.

Applied and Environmental Microbiology, April 2005, p. 2079-2085, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2079-2085.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Langlois, R. J., Hummer, D., LaRoche, J. (2008). Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean. Appl. Environ. Microbiol. 74: 1922-1931 [Abstract] [Full Text]  
  • Bostrom, K. H., Riemann, L., Zweifel, U. L., Hagstrom, A. (2007). Nodularia sp. nifH gene transcripts in the Baltic Sea proper. J PLANKTON RES 29: 391-399 [Abstract] [Full Text]  


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