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Applied and Environmental Microbiology, April 2005, p. 2158-2161, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2158-2161.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Detection of Salmonella Strains and Escherichia coli O157:H7 in Feces of Small Ruminants and Their Isolation with Various Media{dagger}

Steven Pao,* Dhartika Patel, Aref Kalantari, Joseph P. Tritschler, Stephan Wildeus, and Brian L. Sayre

Agricultural Research Station, Virginia State University, Petersburg, Virginia

Received 11 July 2004/ Accepted 9 November 2004

Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.

* Corresponding author. Mailing address: Virginia State University, Agricultural Research Station, P.O. Box 9061, Petersburg, VA 23806. Phone: (804) 524-6715. Fax: (804) 524-5186. E-mail: spao{at}vsu.edu.

{dagger} Contribution of the Virginia State University Research Station (Journal Article Series no. 236).

Applied and Environmental Microbiology, April 2005, p. 2158-2161, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2158-2161.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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