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Applied and Environmental Microbiology, April 2005, p. 2162-2169, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2162-2169.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Comparative Analysis of Bacterial Diversity in Freshwater Sediment of a Shallow Eutrophic Lake by Molecular and Improved Cultivation-Based Techniques{dagger}

Hideyuki Tamaki,1 Yuji Sekiguchi,1 Satoshi Hanada,1 Kazunori Nakamura,1 Nakao Nomura,2 Masatoshi Matsumura,2 and Yoichi Kamagata1*

Institute of Applied Biochemistry, University of Tsukuba,2 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan1

Received 2 April 2004/ Accepted 13 October 2004

Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses.

* Corresponding author. Mailing address: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. Phone: 81-29-861-6591. Fax: 81-29-861-6587. E-mail: y.kamagata{at}aist.go.jp. .

{dagger} Supplemental material for this article can be found at http://aem.asm.org

Applied and Environmental Microbiology, April 2005, p. 2162-2169, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2162-2169.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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