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Applied and Environmental Microbiology, April 2005, p. 2179-2182, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2179-2182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Characterization of the Export of Bulk Poly(A)+ mRNA in Saccharomyces cerevisiae during the Wine-Making Process

Shingo Izawa,1* Reiko Takemura,1 Takeo Miki,2 and Yoshiharu Inoue1

Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto,1 The Institute of Enology and Viticulture, Yamanashi University, Kofu, Yamanashi, Japan2

Received 15 June 2004/ Accepted 19 October 2004

Ethanol stress affects the nuclear export of mRNA similarly to heat shock in Saccharomyces cerevisiae. However, we have little information about mRNA transport in actual alcoholic fermentation. Here we characterized the transport of mRNA during wine making and found that bulk poly(A)+ mRNA accumulated in the nucleus as fermentation progressed.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. Phone: 81 774 38 3731. Fax: 81 774 33 3004. E-mail: izawa{at}kais.kyoto-u.ac.jp.

Applied and Environmental Microbiology, April 2005, p. 2179-2182, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2179-2182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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