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Applied and Environmental Microbiology, April 2005, p. 2190-2194, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2190-2194.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR

Morgan Guilbaud,1 Pierre de Coppet,2 Fabrice Bourion,3 Cinta Rachman,1 Hervé Prévost,1* and Xavier Dousset1

Laboratoire de Microbiologie Alimentaire et Industrielle, ENITIAA,1 Unité des Fonctions Digestives et Nutrition Humaine, INRA, Nantes,2 Association ASEPT, Laval, France3

Received 3 June 2004/ Accepted 9 November 2004

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 102 CFU/cm2.

* Corresponding author. Mailing address: QM2A, LMAI, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France. Phone: 33-2-51785524. Fax: 33-2-51785520. E-mail: prevost{at}enitiaa-nantes.fr.

Applied and Environmental Microbiology, April 2005, p. 2190-2194, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2190-2194.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pan, Y., Breidt, F. Jr. (2007). Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells. Appl. Environ. Microbiol. 73: 8028-8031 [Abstract] [Full Text]  
  • Bruhn, J. B., Haagensen, J. A. J., Bagge-Ravn, D., Gram, L. (2006). Culture Conditions of Roseobacter Strain 27-4 Affect Its Attachment and Biofilm Formation as Quantified by Real-Time PCR. Appl. Environ. Microbiol. 72: 3011-3015 [Abstract] [Full Text]  


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