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Applied and Environmental Microbiology, May 2005, p. 2232-2238, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2232-2238.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Unique Type II Polyketide Synthase Genes in Soil

Biotechnology Center for Agriculture and the Environment,¹ Institute of Marine and Coastal Sciences,² Department of Environmental Sciences,³ Department of Microbiology and Biochemistry, Rutgers University, Cook College, New Brunswick, New Jersey⁴

Received 2 September 2004/ Accepted 30 November 2004

Many bacteria, particularly actinomycetes, are known to produce secondary metabolites synthesized by polyketide synthases (PKS). Bacterial polyketides are a particularly rich source of bioactive molecules, many of which are of potential pharmaceutical relevance. To directly access PKS gene diversity from soil, we developed degenerate PCR primers for actinomycete type II KS (ketosynthase) genes. Twenty-one soil samples were collected from diverse sources in New Jersey, and their bacterial communities were compared by terminal restriction fragment length polymorphism (TRFLP) analysis of PCR products generated using bacterial 16S rRNA gene primers (27F and 1525R) as well as an actinomycete-specific forward primer. The distribution of actinomycetes was highly variable but correlated with the overall bacterial species composition as determined by TRFLP. Two samples were identified to contain a particularly rich and unique actinomycete community based on their TRFLP patterns. The same samples also contained the greatest diversity of KS genes as determined by TRFLP analysis of KS PCR products. KS PCR products from these and three additional samples with interesting TRFLP pattern were cloned, and seven novel clades of KS genes were identified. Greatest sequence diversity was observed in a sample containing a moderate number of peaks in its KS TRFLP. The nucleotide sequences were between 74 and 81% identical to known sequences in GenBank. One cluster of sequences was most similar to the KS involved in ardacin (glycopeptide antibiotic) production by Kibdelosporangium aridum. The remaining sequences showed greatest similarity to the KS genes in pathways producing the angucycline-derived antibiotics simocyclinone, pradimicin, and jasomycin.

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