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Applied and Environmental Microbiology, May 2005, p. 2391-2402, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2391-2402.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Adaptation of Corynebacterium glutamicum to Ammonium Limitation: a Global Analysis Using Transcriptome and Proteome Techniques{dagger}

Maike Silberbach,1 Mathias Schäfer,2 Andrea T. Hüser,3 Jörn Kalinowski,3 Alfred Pühler,4 Reinhard Krämer,1 and Andreas Burkovski1*

Institut für Biochemie der Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany,1 Institut für Organische Chemie der Universität zu Köln, Greinstrasse 4, D-50939 Köln, Germany,2 Institut für Genomforschung, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany,3 Lehrstuhl für Genetik, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany4

Received 22 September 2004/ Accepted 1 December 2004

Theresponse of Corynebacterium glutamicum to ammonium limitation was studied by transcriptional and proteome profiling of cells grown in a chemostat. Our results show that ammonium-limited growth of C. glutamicum results in a rearrangement of the cellular transport capacity, changes in metabolic pathways for nitrogen assimilation, amino acid biosynthesis, and carbon metabolism, as well as a decreased cell division. Since transcription at different growth rates was studied, it was possible to distinguish specific responses to ammonium limitation and more general, growth rate-dependent alterations in gene expression. The latter include a number of genes encoding ribosomal proteins and genes for FoF1-ATP synthase subunits.

* Corresponding author. Mailing address: Institut für Biochemie, Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany. Phone: 49-221-470-6472. Fax: 49-221-470-5091. E-mail: a.burkovski{at}uni-koeln.de.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2005, p. 2391-2402, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2391-2402.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Vertes, A. A., Inui, M., Yukawa, H. (2005). Manipulating Corynebacteria, from Individual Genes to Chromosomes. Appl. Environ. Microbiol. 71: 7633-7642 [Full Text]  


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