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Applied and Environmental Microbiology, May 2005, p. 2460-2466, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2460-2466.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Distribution of Genes for Synthesis of Trehalose and Mannosylglycerate in Thermus spp. and Direct Correlation of These Genes with Halotolerance

Susana Alarico,1 Nuno Empadinhas,1 Catarina Simões,1 Zélia Silva,1 Anke Henne,2 Ana Mingote,3 Helena Santos,3 and Milton S. da Costa1*

Departamento de Bioquímica and Centro de Neurociências e Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal,1 Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetik, Grisebachstr. 8, 37077 Göttingen, Germany,2 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 Oeiras, Portugal3

Received 5 August 2004/ Accepted 30 November 2004

In this study we correlate the presence of genes leading to the synthesis of trehalose and mannosylglycerate (MG) in 17 strains of the genus Thermus with the ability of the strains to grow and accumulate these compatible solutes in a defined medium containing NaCl. The two sets of genes, namely, otsA/otsB for the synthesis of trehalose and mpgS/mpgP for the synthesis of MG, were necessary for the growth of Thermus thermophilus in a defined medium containing up to 6% NaCl. Strains lacking a complete otsA gene did not grow in defined medium containing >2% NaCl. One strain of T. thermophilus lacking the genes for the synthesis of MG did not grow in a medium with >1% NaCl. We did not identify any of these genes in the type strains of the other seven species of Thermus, and none of those strains grew in defined medium with 1% NaCl. The results strongly indicate that the combined accumulation of trehalose and MG is required for optimal osmotic adjustment.

* Corresponding author. Mailing address: Departamento de Bioquímica and Centro de Neurociências e Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal. Phone: 351-239 824024. Fax: 351-239 826798. E-mail: milton{at}ci.uc.pt.

Applied and Environmental Microbiology, May 2005, p. 2460-2466, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2460-2466.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alarico, S., da Costa, M. S., Empadinhas, N. (2008). Molecular and Physiological Role of the Trehalose-Hydrolyzing {alpha}-Glucosidase from Thermus thermophilus HB27. J. Bacteriol. 190: 2298-2305 [Abstract] [Full Text]  
  • Costa, J., Empadinhas, N., da Costa, M. S. (2007). Glucosylglycerate Biosynthesis in the Deepest Lineage of the Bacteria: Characterization of the Thermophilic Proteins GpgS and GpgP from Persephonella marina. J. Bacteriol. 189: 1648-1654 [Abstract] [Full Text]  
  • Costa, J., Empadinhas, N., Goncalves, L., Lamosa, P., Santos, H., da Costa, M. S. (2006). Characterization of the Biosynthetic Pathway of Glucosylglycerate in the Archaeon Methanococcoides burtonii. J. Bacteriol. 188: 1022-1030 [Abstract] [Full Text]  


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