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Applied and Environmental Microbiology, May 2005, p. 2762-2765, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2762-2765.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

The Gene yggE Functions in Restoring Physiological Defects of Escherichia coli Cultivated under Oxidative Stress Conditions

SunYoung Kim,¹ Motomu Nishioka,¹ Shuhei Hayashi,^2, Hiroyuki Honda,² Takeshi Kobayashi,^2, and Masahito Taya^1,3*

Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531,¹ Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Aichi 464-8603,² Research Center for Solar Energy Chemistry, Osaka University, Toyonaka, Osaka 560-8531, Japan³

Received 13 September 2004/ Accepted 24 November 2004

DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO₂, and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 vector. The content of reactive oxygen species (ROS) in IM303 (I4) cells carrying pYGE was reduced to 31% of ROS content in the control cells with pUC 19. In the culture of wild-type strain, E. coli MM294, in the medium with paraquat (10 µmol/l), maximum specific growth rate of the cells with pYGE was about five times higher than that of the control cells, with a decreased ROS content in the former cells. The introduction of pYGE also suppressed the occurrence of the cells with altered amino acid requirement in the culture of MM294 cells with paraquat.

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* Corresponding author. Mailing address: Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan. Phone: 81-6-6850-6251. Fax: 81-6-6850-6254. E-mail: taya{at}cheng.es.osaka-u.ac.jp.

Present address: Department of Applied Life Science, School of Engineering, Sojo University, Kumamoto 860-0082, Japan.

Present address: Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Aichi 487-8501, Japan.

Supplemental material for this article may be found at http://aem.asm.org/.

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Applied and Environmental Microbiology, May 2005, p. 2762-2765, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2762-2765.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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