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Applied and Environmental Microbiology, May 2005, p. 2771-2776, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2771-2776.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes{dagger}

Cédric F. V. Hobel,1 Viggó T. Marteinsson,1* Gudmundur Ó. Hreggvidsson,1,2 and Jakob K. Kristjánsson1

Prokaria Ltd., Gylfaflöt 5, IS-112 Reykjavik,1 Institute of Biology, University of Iceland, Sturlugata 7, IS-101 Reykjavik, Iceland2

Received 7 July 2004/ Accepted 2 December 2004

The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach.

* Corresponding author. Mailing address: Prokaria Ltd., Gylfaflöt 5, IS-112 Reykjavik, Iceland. Phone: (354) 570 7900. Fax: (354) 570 7901. E-mail: viggo{at}prokaria.com.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2005, p. 2771-2776, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2771-2776.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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