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SHORT REPORT

Lactococcus lactis SpOx Spontaneous Mutants: a Family of Oxidative-Stress-Resistant Dairy Strains

Tatiana Rochat ,^1,, Jean-Jacques Gratadoux,^1,, Gérard Corthier,² Bérard Coqueran,² Maria-Elena Nader-Macias,³ Alexandra Gruss,¹ and Philippe Langella^1*,

Unité de Recherches Laitières et de Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France,¹ Unité d'Ecologie et Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France,² Centro de Referencia para Lactobacilos (CERELA-CONICET) Chacabuco 145, Tucuman 4000, Argentina³

Received 16 July 2004/ Accepted 23 November 2004

Numerous industrial bacteria generate hydrogen peroxide (H₂O₂), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H₂O₂. Three SpOx mutants displayed greater H₂O₂ resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H₂O₂-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.

This work is dedicated to Patrick Duwat, who died on 5 January 2000.

These authors contributed equally to this work.

Present address: Unité d'Ecologie et Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France.

This article has been cited by other articles:

• Rochat, T., Miyoshi, A., Gratadoux, J. J., Duwat, P., Sourice, S., Azevedo, V., Langella, P. (2005). High-level resistance to oxidative stress in Lactococcus lactis conferred by Bacillus subtilis catalase KatE. Microbiology 151: 3011-3018 [Abstract] [Full Text]