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Applied and Environmental Microbiology, May 2005, p. 2789-2792, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2789-2792.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetically Engineered Wine Yeast Produces a High Concentration of L-Lactic Acid of Extremely High Optical Purity

Satoshi Saitoh,^1, Nobuhiro Ishida,^2,* Toru Onishi,¹ Kenro Tokuhiro,² Eiji Nagamori,² Katsuhiko Kitamoto,³ and Haruo Takahashi²

Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co., Miyoshi-cho, Aichi 470-0201,¹ Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192,² Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan³

Received 26 August 2004/ Accepted 28 November 2004

For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.

These two authors contributed equally to this work.

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