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Applied and Environmental Microbiology, June 2005, p. 2848-2852, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.2848-2852.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cultivation System Using Glass Beads Immersed in Liquid Medium Facilitates Studies of Streptomyces Differentiation

Liem D. Nguyen,1,2,{dagger} Ladislava Kalachová,1 Jana Novotná,1 Martin Holub,1 Olga Kofronová,1 Oldrich Benada,1 Charles J. Thompson,2,{dagger} and Jaroslav Weiser1*

Institute of Microbiology, Czech Academy of Sciences, 14220 Prague 4, Czech Republic,1 Biozentrum, University of Basel, 4056 Basel, Switzerland2

Received 13 August 2004/ Accepted 16 December 2004

A two-phase cultivation system was developed which will enable studies of streptomycete differentiation by molecular biological and global techniques such as transcriptomics and proteomics. The system is based on a solid phase formed by glass beads corresponding to particles in soil, clay, or sand natural habitats of streptomycetes. The beads are immersed in a liquid medium that allows easy modification or replacement of nutrients and growth factors as well as radioactive labeling of proteins. Scanning electron microscopy was used to analyze morphological differentiation of streptomycetes on glass beads and two-dimensional protein electrophoresis to demonstrate the potential of the system for analyses of protein synthesis profiles during the developmental program. This system facilitates studies of differentiation including expression and posttranslation modifications of streptomycetes proteins, secondary metabolite biosynthesis, and morphological development.

* Corresponding author. Mailing address: Institute of Microbiology, Czech Academy of Sciences, 14220 Prague 4, Czech Republic. Phone: 420-241062389. Fax: 420-241722257. E-mail: weiser{at}biomed.cas.cz.

{dagger} Present address: Department of Microbiology and Immunology, 300-6174 University Blvd., University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.

Applied and Environmental Microbiology, June 2005, p. 2848-2852, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.2848-2852.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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