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Applied and Environmental Microbiology, June 2005, p. 3171-3178, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3171-3178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Movement and Fixation of Intestinal Microbiota after Administration of Human Feces to Germfree Mice

Ryoko Kibe,1,2* Mitsuo Sakamoto,2 Hiroshi Yokota,1 Hiroki Ishikawa,3 Yuji Aiba,3,4 Yasuhiro Koga,3 and Yoshimi Benno2

Department of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai-midorimachi, Ebetsu, Hokkaido 069-8501, Japan,1 Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan,2 Department of Infectious Diseases, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1100, Japan,3 Sagami Research Laboratories, Wakamoto Pharmaceutical Co., Ltd., 378 Kanade, Ohimachi, Ashigarakami-gun, Kanagawa 258-0018, Japan4

Received 8 October 2004/ Accepted 20 December 2004

Human flora-associated (HFA) mice have been considered a tool for studying the ecology and metabolism of intestinal bacteria in humans, although they have some limitations as a model. Shifts in dominant species of microbiota in HFA mice after the administration of human intestinal microbiota was revealed by 16S rRNA gene sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses. Characteristic terminal restriction fragments (T-RFs) were quantified as the proportion of total peak area of all T-RFs. Only the proportion of the T-RF peak at bp 366, identified as the Gammmaproteobacteria group and the family Coriobacteriaceae, was reduced in this study. Increased T-RFs over time at bp 56, 184, and 196 were affiliated with the Clostridium group. However, most of the isolated bacteria with unique population shifts were phylotypes. The vertical transmission of the intestinal microbiota of the mouse offspring was also investigated by dendrogram analysis derived from the similarity of T-RFLP patterns among samples. As a result, the intestinal microbiota of HFA mice and their offspring reflected the composition of individual human intestinal bacteria with some modifications. Moreover, we revealed that human-derived lactobacilli (HDL), which have been considered difficult to colonize in the HFA mouse intestine in previous studies based on culture methods, could be detected in the HFA mouse intestine by using a lactic acid bacterium-specific primer and HDL-specific primers. Our results indicate that the intestinal microbiota of HFA mice represents a limited sample of bacteria from the human source and are selected by unknown interactions between the host and bacteria.

* Corresponding author. Mailing address: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResouce Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Phone: 81-48-467-9562. Fax: 81-48-462-4619. E-mail: r-kibe{at}jcm.riken.jp.

Applied and Environmental Microbiology, June 2005, p. 3171-3178, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3171-3178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakanishi, Y., Murashima, K., Ohara, H., Suzuki, T., Hayashi, H., Sakamoto, M., Fukasawa, T., Kubota, H., Hosono, A., Kono, T., Kaminogawa, S., Benno, Y. (2006). Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides. Appl. Environ. Microbiol. 72: 6271-6276 [Abstract] [Full Text]  


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