This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paul, J. H. Articles by Patterson, S. Search for Related Content PubMed PubMed Citation Articles by Paul, J. H. Articles by Patterson, S. Agricola Articles by Paul, J. H. Articles by Patterson, S.

Complete Genome Sequence of HSIC, a Pseudotemperate Marine Phage of Listonella pelagia

University of South Florida, 140 7th Ave. South, St. Petersburg, Florida 33701,¹ University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way, Newark, Delaware 19711,² Department of Biology, San Diego State University, San Diego, California 92182-4614,³ Center for Microbial Sciences, San Diego State University, San Diego, California 92182-4614,⁴ Proteomics Core Facility, Moffitt Cancer Research Center, 12902 Magnolia Drive, Tampa, Florida 33612⁵

Received 11 October 2004/ Accepted 31 December 2004

The genome for the marine pseudotemperate member of the Siphoviridae HSIC has been sequenced using a combination of linker amplification library construction, restriction digest library construction, and primer walking. HSIC enters into a pseudolysogenic relationship with its host, Listonella pelagia, characterized by sigmoidal growth curves producing >10⁹ cells/ml and >10¹¹ phage/ml. The genome (37,966 bp; G+C content, 44%) contained 47 putative open reading frames (ORFs), 17 of which had significant BLASTP hits in GenBank, including a ß subunit of DNA polymerase III, a helicase, a helicase-like subunit of a resolvasome complex, a terminase, a tail tape measure protein, several phage-like structural proteins, and 1 ORF that may assist in host pathogenicity (an ADP ribosyltransferase). The genome was circularly permuted, with no physical ends detected by sequencing or restriction enzyme digestion analysis, and lacked a cos site. This evidence is consistent with a headful packaging mechanism similar to that of Salmonella phage P22 and Shigella phage Sf6. Because none of the phage-like ORFs were closely related to any existing phage sequences in GenBank (i.e., none more than 62% identical and most <25% identical at the amino acid level), HSIC is unique among phages that have been sequenced to date. These results further emphasize the need to sequence phages from the marine environment, perhaps the largest reservoir of untapped genetic information.

This article has been cited by other articles:

• Schoenfeld, T., Patterson, M., Richardson, P. M., Wommack, K. E., Young, M., Mead, D. (2008). Assembly of Viral Metagenomes from Yellowstone Hot Springs. Appl. Environ. Microbiol. 74: 4164-4174 [Abstract] [Full Text]  
• Mobberley, J. M., Authement, R. N., Segall, A. M., Paul, J. H. (2008). The Temperate Marine Phage {Phi}HAP-1 of Halomonas aquamarina Possesses a Linear Plasmid-Like Prophage Genome. J. Virol. 82: 6618-6630 [Abstract] [Full Text]  
• Yoshida, T., Nagasaki, K., Takashima, Y., Shirai, Y., Tomaru, Y., Takao, Y., Sakamoto, S., Hiroishi, S., Ogata, H. (2008). Ma-LMM01 Infecting Toxic Microcystis aeruginosa Illuminates Diverse Cyanophage Genome Strategies. J. Bacteriol. 190: 1762-1772 [Abstract] [Full Text]  
• Thompson, F. L., Klose, K. E., the AVIB Group, (2006). Vibrio2005: the First International Conference on the Biology of Vibrios. J. Bacteriol. 188: 4592-4596 [Full Text]