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Applied and Environmental Microbiology, June 2005, p. 3337-3341, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3337-3341.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Surface Display of Recombinant Proteins on Bacillus thuringiensis Spores

Cheng Du,1* Wing C. Chan,1 Timothy W. McKeithan,2 and Kenneth W. Nickerson3

Department of Pathology and Microbiology,1 Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198,2 School of Biological Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska 685883

Received 31 August 2004/ Accepted 15 December 2004

The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198. Phone: (402) 559-7921. Fax: (402) 559-4077. E-mail: cdu{at}unmc.edu.

Applied and Environmental Microbiology, June 2005, p. 3337-3341, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3337-3341.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Kwon, S. J., Jung, H.-C., Pan, J.-G. (2007). Transgalactosylation in a Water-Solvent Biphasic Reaction System with {beta}-Galactosidase Displayed on the Surfaces of Bacillus subtilis Spores. Appl. Environ. Microbiol. 73: 2251-2256 [Abstract] [Full Text]  


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