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Applied and Environmental Microbiology, June 2005, p. 3342-3347, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3342-3347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genomic DNA Amplification from a Single Bacterium

Arumugham Raghunathan,{dagger} Harley R. Ferguson Jr., Carole J. Bornarth,{dagger} Wanmin Song, Mark Driscoll,{ddagger} and Roger S. Lasken*

Molecular Staging, Inc., New Haven, Connecticut

Received 3 August 2004/ Accepted 20 December 2004

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using {phi} 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.

* Corresponding author. Present address: Allegheny-Singer Research Institute, 320 East North Ave., Pittsburgh, PA 15212-4772. Phone: (412) 359-6214. Fax: (412) 359-8169. E-mail: rlasken2000{at}Yahoo.com.

{dagger} Present address: QIAGEN Inc., 27220 Turnberry Lane, Valencia, CA 91355.

{ddagger} Present address: 454 Corporation, New Haven, Conn.

Applied and Environmental Microbiology, June 2005, p. 3342-3347, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3342-3347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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