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Applied and Environmental Microbiology, June 2005, p. 3359-3363, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3359-3363.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Development and Evaluation of a Broadly Reactive TaqMan Assay for Rapid Detection of Hepatitis A Virus

N. Jothikumar,1,2* T. L. Cromeans,1,2 M. D. Sobsey,1 and B. H. Robertson2

Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7431,1 Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 3 September 2004/ Accepted 20 December 2004

Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.

* Corresponding author. Present address: Department of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA 30341. Phone: (770) 488-3775. Fax: (770) 488-4253. E-mail: JIN2{at}cdc.gov.

Applied and Environmental Microbiology, June 2005, p. 3359-3363, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3359-3363.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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