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Applied and Environmental Microbiology, June 2005, p. 3379-3383, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3379-3383.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nitrate and Phosphate Affect Cultivability of Cyanobacteria from Environments with Low Nutrient Levels{dagger}

Anneliese Ernst,1* Manfred Deicher,2 Peter M. J. Herman,1 and Ute I. A. Wollenzien1

Centre for Estuarine and Marine Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 4400 AC Yerseke, The Netherlands,1 Fachbereich Physik, Universität Konstanz, 78457 Konstanz, Germany2

Received 16 August 2004/ Accepted 15 December 2004

Nitrate and phosphate concentrations higher than those found in the natural environment slowed down growth of two strains of non-bloom-forming, phycoerythrin-rich Synechococcus spp. isolated from mesotrophic subalpine lakes. The results make clear why isolation of these picocyanobacteria in standard cultivation media was difficult. At low concentrations, closely related strains exhibited distinct growth characteristics with respect to these two nutrients, possibly explaining differences in their seasonal appearance in the natural environment.


* Corresponding author. Mailing address: NIOO-CEME, P.O. Box 140, NL-4400 AC Yerseke, The Netherlands. Phone: 31 (0) 113 577300. Fax: 31 (0) 113 573616. E-mail: a.ernst{at}nioo.knaw.nl.

{dagger} This is publication 3506 NIOO-KNAW of the Netherlands Institute of Ecology.


Applied and Environmental Microbiology, June 2005, p. 3379-3383, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3379-3383.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.