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Applied and Environmental Microbiology, June 2005, p. 3384-3387, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3384-3387.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Evidence for the Presence of an Alternative Glucose Transport System in Clostridium beijerinckii NCIMB 8052 and the Solvent-Hyperproducing Mutant BA101

Jieun Lee,1 Wilfrid J. Mitchell,2 Martin Tangney,3 and H. P. Blaschek1*

Food Microbiology Division, Department of Food Science and Human Nutrition, University of Illinois, 1207 W. Gregory Drive, Urbana, Illinois 61801,1 School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, United Kingdom,2 School of Life Sciences, Napier University, Merchiston Campus, Edinburgh EH10 5DT, United Kingdom3

Received 13 May 2004/ Accepted 29 December 2004

The effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by Clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a non-PTS system probably energized by the transmembrane proton gradient. In both wild-type C. beijerinckii NCIMB 8052 and the butanol-hyperproducing mutant BA101, PTS activity declined at the end of exponential growth, while glucokinase activity increased in the later stages of fermentation. The non-PTS uptake system, together with enhanced glucokinase activity, may provide an explanation for the ability of the mutant to utilize glucose more effectively during fermentation despite the fact that it is partially defective in PTS activity.

* Corresponding author. Mailing address: 1207 W. Gregory Drive, Urbana, IL 61801. Phone: (217) 333-8224. Fax: (217) 244-2517. E-mail: blaschek{at}uiuc.edu.

Applied and Environmental Microbiology, June 2005, p. 3384-3387, Vol. 71, No. 6
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.6.3384-3387.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shi, Z., Blaschek, H. P. (2008). Transcriptional Analysis of Clostridium beijerinckii NCIMB 8052 and the Hyper-Butanol-Producing Mutant BA101 during the Shift from Acidogenesis to Solventogenesis. Appl. Environ. Microbiol. 74: 7709-7714 [Abstract] [Full Text]  
  • Han, B., Liu, H., Hu, X., Cai, Y., Zheng, D., Yuan, Z. (2007). Molecular Characterization of a Glucokinase with Broad Hexose Specificity from Bacillus sphaericus Strain C3-41. Appl. Environ. Microbiol. 73: 3581-3586 [Abstract] [Full Text]  


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