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Applied and Environmental Microbiology, July 2005, p. 3761-3769, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3761-3769.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Enhanced Trehalose Production Improves Growth of Escherichia coli under Osmotic Stress{dagger}

J. E. Purvis, L. P. Yomano, and L. O. Ingram*

Department of Microbiology and Cell Science, Box 110700, University of Florida, Gainesville, Florida 32611

Received 7 July 2004/ Accepted 9 January 2005

The biosynthesis of trehalose has been previously shown to serve as an important osmoprotectant and stress protectant in Escherichia coli. Our results indicate that overproduction of trehalose (integrated lacI-Ptac-otsBA) above the level produced by the native regulatory system can be used to increase the growth of E. coli in M9-2% glucose medium at 37°C to 41°C and to increase growth at 37°C in the presence of a variety of osmotic-stress agents (hexose sugars, inorganic salts, and pyruvate). Smaller improvements were noted with xylose and some fermentation products (ethanol and pyruvate). Based on these results, overproduction of trehalose may be a useful trait to include in biocatalysts engineered for commodity chemicals.

* Corresponding author. Mailing address: Dept. of Microbiology and Cell Science, Bldg. 981 Museum Road, IFAS, P.O. Box 110700, University of Florida, Gainesville, FL 32611. Phone: (352) 392-8176. Fax: (352) 846-0969. E-mail: ingram{at}ufl.edu.

{dagger} Florida Agricultural Experiment Station publication no. R-10904.

Applied and Environmental Microbiology, July 2005, p. 3761-3769, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3761-3769.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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