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Applied and Environmental Microbiology, July 2005, p. 3826-3831, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3826-3831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Diversity of Methanotrophic Bacteria in Tropical Upland Soils under Different Land Uses

Claudia Knief,1,2 Supika Vanitchung,3 Narumon W. Harvey,3 Ralf Conrad,1 Peter F. Dunfield,1,4 and Amnat Chidthaisong3*

Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße, 35043 Marburg, Germany,1 Laboratoire des Interactions Plantes Micro-organismes, INRA/CNRS, BP 52627, Chemin de Borde Rouge, 31326 Castanat-Tolosan, France,2 Joint Graduate School of Energy and Environment, King Mongkut's University of Technology Thonburi, 91 Pracha-Uthit Rd., Bangkok 10140, Thailand,3 Institute of Geological and Nuclear Sciences, Wairakei Research Centre, 114 Karetoto Rd., Taupo, New Zealand 164

Received 10 October 2004/ Accepted 2 February 2005

Three upland soils from Thailand, a natural forest, a 16-year-old reforested site, and an agricultural field, were studied with regard to methane uptake and the community composition of methanotrophic bacteria (MB). The methane uptake rates were similar to rates described previously for forest and farmland soils of the temperate zone. The rates were lower at the agricultural site than at the native forest and reforested sites. The sites also differed in the MB community composition, which was characterized by denaturing gradient gel electrophoresis (DGGE) of pmoA gene fragments (coding for a subunit of particulate methane monooxygenase) that were PCR amplified from total soil DNA extracts. Cluster analysis based on the DGGE banding patterns indicated that the MB communities at the forested and reforested sites were similar to each other but different from that at the farmland site. Sequence analysis of excised DGGE bands indicated that Methylobacter spp. and Methylocystis spp. were present. Sequences of the "forest soil cluster" or "upland soil cluster {alpha}," which is postulated to represent organisms involved in atmospheric methane consumption in diverse soils, were detected only in samples from the native forest and reforested sites. Additional sequences that may represent uncultivated groups of MB in the Gammaproteobacteria were also detected.

* Corresponding author. Mailing address: Joint Graduate School of Energy and Environment, King Mongkut's University of Technology Thonburi, 91 Pracha-Uthit Rd., Bangkok 10140, Thailand. Phone: 66-2-4708309. Fax: 66-2-8729805. E-mail: amnat_c{at}jgsee.kmutt.ac.th.

Applied and Environmental Microbiology, July 2005, p. 3826-3831, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3826-3831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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