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Applied and Environmental Microbiology, July 2005, p. 3872-3881, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3872-3881.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effects of Subtherapeutic Administration of Antimicrobial Agents to Beef Cattle on the Prevalence of Antimicrobial Resistance in Campylobacter jejuni and Campylobacter hyointestinalis{dagger}

G. D. Inglis,1* T. A. McAllister,1 H. W. Busz,1 L. J. Yanke,1 D. W. Morck,2 M. E. Olson,3 and R. R. Read3

Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta,1 Department of Biological Sciences, University of Calgary, Calgary, Alberta,2 Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada3

Received 2 September 2004/ Accepted 6 January 2005

The influence of antimicrobial agents on the development of antimicrobial resistance (AMR) in Campylobacter isolates recovered from 300 beef cattle maintained in an experimental feedlot was monitored over a 315-day period (11 sample times). Groups of calves were assigned to one of the following antimicrobial treatments: chlortetracycline and sulfamethazine (CS), chlortetracycline alone (Ct), virginiamycin, monensin, tylosin phosphate, and no antimicrobial agent (i.e., control treatment). In total, 3,283 fecal samples were processed for campylobacters over the course of the experiment. Of the 2,052 bacterial isolates recovered, 92% were Campylobacter (1,518 were Campylobacter hyointestinalis and 380 were C. jejuni). None of the antimicrobial treatments decreased the isolation frequency of C. jejuni relative to the control treatment. In contrast, C. hyointestinalis was isolated less frequently from animals treated with CS and to a lesser extent from animals treated with Ct. The majority (≥94%) of C. jejuni isolates were sensitive to ampicillin, erythromycin, and ciprofloxacin, but more isolates with resistance to tetracycline were recovered from animals fed Ct. All of the 1,500 isolates of C. hyointestinalis examined were sensitive to ciprofloxacin. In contrast, 11%, 10%, and 1% of these isolates were resistant to tetracycline, erythromycin, and ampicillin, respectively. The number of animals from which C. hyointestinalis isolates with resistance to erythromycin and tetracycline were recovered differed among the antimicrobial treatments. Only Ct administration increased the carriage rates of erythromycin-resistant isolates of C. hyointestinalis, and the inclusion of CS in the diet increased the number of animals from which tetracycline-resistant isolates were recovered. The majority of C. hyointestinalis isolates with resistance to tetracycline were obtained from cohorts within a single pen, and most of these isolates were recovered from cattle during feeding of a forage-based diet as opposed to a grain-based diet. The findings of this study show that the subtherapeutic administration of tetracycline, alone and in combination with sulfamethazine, to feedlot cattle can select for the carriage of resistant strains of Campylobacter species. Considering the widespread use of in-feed antimicrobial agents and the high frequency of beef cattle that shed campylobacters, the development of AMR should be monitored as part of an on-going surveillance program.

* Corresponding author. Mailing address: Agriculture & Agri-Food Canada Research Centre, 5403 1st Avenue S, Lethbridge, AB T1J 4B1, Canada. Phone: (403) 317-3355. Fax: (403) 382-3156. E-mail: inglisd{at}agr.gc.ca.

{dagger} Contribution 04044 from the Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta, Canada.

Applied and Environmental Microbiology, July 2005, p. 3872-3881, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3872-3881.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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