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Applied and Environmental Microbiology, July 2005, p. 4052-4056, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4052-4056.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mediterranean Fruit Fly as a Potential Vector of Bacterial Pathogens

Shlomo Sela,1* David Nestel,2 Riky Pinto,1 Esther Nemny-Lavy,2 and Moshe Bar-Joseph3

Department of Food Sciences, Institute for Technology and Storage of Fresh Produce,1 Department of Entomology,2 Department of Virology, Institute of Plant Protection, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan, Israel3

Received 26 October 2004/ Accepted 1 February 2005

The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop eggs. After reaching sexual maturity and copulating, female flies lay eggs in fruit by puncturing the skin with their ovipositors and injecting batches of eggs into the wounds. In view of the increase in food-borne illnesses associated with consumption of fresh produce and unpasteurized fruit juices, we investigated the potential of Mediterranean fruit fly to serve as a vector for transmission of human pathogens to fruits. Addition of green fluorescent protein (GFP)-tagged Escherichia coli to a Mediterranean fruit fly feeding solution resulted in a dose-dependent increase in the fly's bacterial load. Flies exposed to fecal material enriched with GFP-tagged E. coli were similarly contaminated and were capable of transmitting E. coli to intact apples in a cage model system. Washing contaminated apples with tap water did not eliminate the E. coli. Flies inoculated with E. coli harbored the bacteria for up to 7 days following contamination. Fluorescence microscopy demonstrated that the majority of fluorescent bacteria were confined along the pseudotrachea in the labelum edge of the fly proboscis. Wild flies captured at various geographic locations were found to carry coliforms, and in some cases presumptive identification of E. coli was made. These findings support the hypothesis that the common Mediterranean fruit fly is a potential vector of human pathogens to fruits.

* Corresponding author. Mailing address: Department of Food Sciences, ARO, The Volcani Center, Beth-Dagan 50250, Israel. Phone: 972-3-9683750. Fax: 972-3-9683692. E-mail: shlomos{at}volcani.agri.gov.il.

Applied and Environmental Microbiology, July 2005, p. 4052-4056, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4052-4056.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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