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Applied and Environmental Microbiology, July 2005, p. 4144-4148, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4144-4148.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Elucidating the Key Member of a Linuron-Mineralizing Bacterial Community by PCR and Reverse Transcription-PCR Denaturing Gradient Gel Electrophoresis 16S rRNA Gene Fingerprinting and Cultivation

Sebastian R. Sørensen,1* Jim Rasmussen,1,2 Carsten S. Jacobsen,1,3 Ole S. Jacobsen,1 René K. Juhler,1 and Jens Aamand1

Department of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen K, Denmark,1 Department of Agricultural Sciences, The Royal Veterinary and Agricultural University (KVL), Højbakkegårds Allé 30, DK-2630 Tåstrup, Denmark,2 Department of Natural Sciences and Department of Ecology, KVL, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark3

Received 26 September 2004/ Accepted 10 January 2005

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N'-methoxy-N'-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U-14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

* Corresponding author. Mailing address: Department of Geochemistry, Geological Survey of Denmark and Greenland, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. Phone: 45 3814 2317. Fax: 45 3814 2050. E-mail: srs{at}geus.dk.

Applied and Environmental Microbiology, July 2005, p. 4144-4148, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4144-4148.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sorensen, S. R., Albers, C. N., Aamand, J. (2008). Rapid Mineralization of the Phenylurea Herbicide Diuron by Variovorax sp. Strain SRS16 in Pure Culture and within a Two-Member Consortium. Appl. Environ. Microbiol. 74: 2332-2340 [Abstract] [Full Text]  
  • Sorensen, S. R., Holtze, M. S., Simonsen, A., Aamand, J. (2007). Degradation and Mineralization of Nanomolar Concentrations of the Herbicide Dichlobenil and Its Persistent Metabolite 2,6-Dichlorobenzamide by Aminobacter spp. Isolated from Dichlobenil-Treated Soils. Appl. Environ. Microbiol. 73: 399-406 [Abstract] [Full Text]  


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