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Applied and Environmental Microbiology, July 2005, p. 4156-4159, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4156-4159.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Effect of an Artificial RNA Marker on Gene Expression in Escherichia coli

Don L. Tucker,1 Fathi Karouia,1 Jim Wang,2 Yi Luo,2 Tong-Bin Li,2 Richard C. Willson,1,3 Yuriy Fofanov,2 and George E. Fox1,3*

Department of Biology and Biochemistry,1 Department of Computer Science,2 Department of Chemical Engineering, University of Houston, Houston, Texas 77204-50013

Received 25 August 2004/ Accepted 4 January 2005

Transcriptional analysis was used to examine the effect of a genomically encoded artificial RNA on Escherichia coli in rich and minimal media. Only the expression of a single gene, deoC, was unequivocally affected under both conditions. E. coli marker strains of this type may be useful in monitoring the fate and transport of bacteria in various applications.

* Corresponding author. Mailing address: Dept. Biology and Biochemistry, University of Houston, Houston, TX 77204-5001. Phone: (713) 743-8363. Fax: (713) 743-8351. E-mail: fox{at}uh.edu.

Applied and Environmental Microbiology, July 2005, p. 4156-4159, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4156-4159.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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